Centro Nacional de Investigaciones Oncológicas CNIO

Contact Information


Phone: (+34) 917328000

Fax: (+34) 917328010.

Address:
C/ Melchor Fernández Almagro nº 3
28029 Madrid
España

Headquarters





Brief description of Institution


The CNIO is a centre of excellence in cancer and innovation research. It is composed by three broad categories:

  • Basic research: Composed by three programs: Molecular oncology, Cancer biology and Structural and Biocomputational biology.
  • Traslational research: Composed by two programs: human cancer genetic and clinic research.
  • Innovation: Composed by the Biotechnology and Experimental therapy programs.

 

Institution


Institution: Centro Nacional de Investigaciones Oncológicas CNIO.

Ownership: Public.

Activity begining year: 2008.

Principal Investigator: Joaquín Pastor.

Contact Person: Joaquín Pastor.

Web: http://www.cnio.es/

Facilities

Usable surface area (m2): 450.

Nuclear Security Council Authorization: Yes.

Animal Facility: Yes.

R&D Department: Yes.

Medical Department: No.

Quality department: No.

Production Department: No.

Chemistry Department: Yes.

Others (please specify): No.

Description: -.


Infraestructure


Experimental Terapuetics Programme at CNIO has a HTS lab equipped with two automated liquid handling instruments BIOMEK FX and Biomek FX Dual Span 8 from Beckman-Coulter. We account with two multiparametric readers Victor II and Envision from Perkin Elmer.

ETP-CNIO's cellular assays are performed using an integrated workstation. The workstation includes a Biomek FX Dual Arm Liquid Handling System with Span-8 Pipettor and Multi-Channel Pipettor linked to a Cytomat microplate hotel, a microplate washer and a Cytomat 2 CO2 incubator. Apart from the multiparmetric plate readers described above we have a High Content Screening (HCS) plate reader (BD Pathway HT, from Beckton Dickinson) for confocal in vivo real time image capturing.

We also accoung with an agilent 1100 liquid chromatographer coupled to an API2000 (Applied Biosystems) triple quadrupole.

ETP-Med Chem acoounts with 4 chemistry labs of aprox. 300 m2 of laboratory space.


Staff


Permanent Staff

Number of PhDs: 17.
PhDs Functions: Diseño y sintesis de compuestos y establecimiento de SAR, caracterización bioquí­mica y farmacológica in vitro e in vivo de los compuestos.

Number of Graduates: 2.
Graduates Functions: Síntesis de compuestos y establecimiento de SAR, caracterización bioquímica de los compuestos..

Number of Diplomates: 0.
Diplomates Functions: -.

Number of Technicians: 0.
Technicians Functions: -.

Other Personnel: 0.
Other Personnel Functions: -.


Recruited Staff

Number of PhDs: 0.
PhDs Functions: -.

Number of Graduates: 0.
Graduates Functions: -.

Number of Diplomates: 0.
Diplomates Functions: -.

Number of Technicians: 0.
Technicians Functions: -.

Other Personnel: 0.
Other Personnel Functions: -.


Quality Control and Certifications


Validation criteria for each tested plate: Determinación de z prima y ventana para cada placa incluyendo siempre estandares internos.

Validation criteria for each screening campaign: Escrutinio a punto simple por duplicado, establecimiento de punto de corte y validación de hits obtenidos mediante dosis respuesta. Selección de hits en función de la potencia..

Validation criteria for concentration-response curves: Homogeneidad en los duplicados, pendiente de la curva , establecimiento de platós superior e inferior..

Concentración - Respuesta: -.

Data analisys software: Activitybase de IDBS.

Result storage and management database: Base de datos interna para controlar el manejo de los compuestos, petición de ensayos, datos bioquímicos,ADMET y celulares e in vivo generados basados en la plataforma Pipepilot.

Audits and certifications: -.


Experience and collaborations

  2010 2011 2012 2013 2014
Regional Research Projects - - - - -
National Research Projects 2 2 3 3 4
International Research Projects - - - - -
Contracts with companies - - - - 2
Screening Campaigns 2 2 1 2 3
Maximun output 2000 2000 2000 2000 2000
Developed/Optimized assays  10 10 10 10 10
Others - - - - -

Consulting services


Consulting services available: No.

Consulting services types: -.

Available assays - Capacities

Services

Radioligand binding assays: No.
List of Radioligand binding assays available: -.

Enzymatic activity assays: No.
List of Enzymatic activity assays available: -.

Second messengers measurement assays: No.
List of Second messengers measurement assays available: -.

Cytochrome assays: No.
List of Cytochrome assays available: -.

Cytotoxicity assay: No.
List of Cytotoxicity assays available: -.

Isolated tissues assays: No.
List of Isolated tissues assays available: -.

Ionic channels assays: No.
List of Ionic channels assays available: -.

High content imaging assays: No.
List of High content imaging assays available: -.

Other assays: No.
List of Other assays available: -.

Collaboration

Radioligand binding assays: No.
List of Radioligand binding assays available: -.

Enzymatic activity assays: Yes.
List of Enzymatic activity assays available: Ensayos de fluorescencia indirecta o polarizada, FRET, HTR.FRET, quimioluminiscencia, enzimáticos acoplados y de binding.

Second messengers measurement assays: Yes.
List of Second messengers measurement assays available: ELISA celular, reportero de luciferasa, medición por western blot de fosforilación.

Cytochrome assays: Yes.
List of Cytochrome assays available: Inhibición a punto simple y dosis respuesta de P450 1A2, 2c19, 2C9, 2D6, 3A4.

Cytotoxicity assay: Yes.
List of Cytotoxicity assays available: Cell titer glo, como agentes únicos o en combinación. Determinación de GI50 y CI.

Isolated tissues assays: Yes.
List of Isolated tissues assays available: PK/PD.

Ionic channels assays: Yes.
List of Ionic channels assays available: HerG binding.

High content imaging assays: No.
List of High content imaging assays available: HCS con BD pathway u Opera, fosforilación, localización y niveles.

Other assays: Yes.
List of Other assays available: Estabilidad en microsomas humanos, de ratón y de rata. Migración.






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